Description
• Detect as little as 150 pg/mL of RFP
• Recognizes TagRFP, TurboRFP, DsRed, mCherry, mKate, mRuby, mBanana, mOrange, mPlum, mStrawberry, and tdTomato
• Simpler and faster than FACS analysis
Most imaging studies of rRFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our RFP ELISA Kit measures RFP fluorescence by ELISA in a standard microplate reader.
Introduction: Red fluorescent protein (DsRed) is a spontaneously fluorescent protein isolated from the Indo-Pacific sea coral, Discosoma striata. It absorbs and emits orange-red light and is well suited for multi-color tagging used in FRET. Since the molecular cloning of RFP cDNA and demonstration of RFP as a functional transgene, RFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. RFP can fuse with proteins of interest without interfering significantly with their assembly and function. Based on the structure of the RFP molecule, many RFP variants have been created with much improved fluorescence emission, or shifted excitation or emission spectra that are well suited for fluorescence microscopy and flow cytometry. Although RFP expression can be easily detected under a fluorescence microscope, RFP fluorescence intensity varies from cell to cell because of the heterogeneity nature of RFP expression. In order to quantitate the RFP expression in cells, FACS analysis is usually required, which is both expensive and time consuming.
MyBioSource's RFP ELISA Kit is an enzyme immunoassay developed for detection and quantitation of RFP or RFP fusion protein in cell or tissue samples. The quantity of RFP or its variants (including TagRFP, TurboRFP, DsRed, tdTomato, mCherry, mKate, mRuby, mBanana, mOrange, mPlum, and mStrawberry) in an unknown sample is determined by comparing its absorbance with that of a known recombinant RFP standard curve. The kit has detection sensitivity limit of 150 pg/mL RFP. The kit also provides an efficient system for rapid quantitation of RFP lentivirus titer for both viral supernatant and purified virus. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and RFP samples